The Effect of Microchimerism on Frequency and Severity of Organ Involvement in Iranian Scleroderma Patients

Document Type: Original Article

Authors

1 Department of Internal Medicine, Division of Rheumatology, Shiraz University of Medical Sciences, Shiraz, Iran

2 Geriatric Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Department of Internal Medicine, Division of Rheumatology, Shiraz University of Medical Sciences, Shiraz, Iran.

4 Autoimmune Disease Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

5 Geriatric Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Objective: Microchimerism is defined by the presence of non-self and circulating cells in a host. Assessing the effect of microchimerism on scleroderma major organ involvements.
Method: This cross sectional study was done on 56 scleroderma patients registered in tertiary rheumatology center of Shiraz University of Medical Sciences. The patients' demographic and disease complications information was gathered by reviewing medical records. Skin score was applied for better assessing skin thickening. High Resolution CT-scan as well as pulmonary function test (PFT) results was also used for investigating pulmonary involvement in patients. The Y chromosome serum levels were measured using Phenol Chloroform Extraction protocol and following real time PCR.
Results: Fifty-six scleroderma patients with mean age of 46 ± 10 years old were recruited in this study (58.9 % with diffused scleroderma and 41.07 % with limited one). Except skin thickening, the most common clinical presentation among the patients was interestitial lung disease (67.8 %). No significant difference was found between the level of Y chromosome in patient with either lung, cardiac, renal or gastrointestinal involvement and ones who did not have these complications. Comparing the serum level of Y chromosome based on results of PFT was also shown to have no significant difference. No association was also demonstrated between serum Y chromosome and skin score.
Conclusion: The serum level of chromosome Y has no impact on severity and frequency of major organ involvements in Iranian scleroderma patients.

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